Research Paper Volume 12, Issue 21 pp 22266—22290

Cholesterol-rich naked mole-rat brain lipid membranes are susceptible to amyloid beta-induced damage in vitro

Figure 1. Mass spectrometry of brain derived total lipid extract. (AD) Quantification of cholesterol (A), sphingomyelin (B), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidylserine (PS) (C), and ceramides (D) in lipid extract from mouse (white bar) or naked mole-rat (black bar) brains. Data are expressed as mean ± SEM. Statistical analysis was performed using the Mann-Whitney test. **p<0.01, significantly different from mouse group. Lipids were extracted from the brains of 7 naked mole-rats and 6 mice (2 independent experiments). (E) Heat map of the different phospholipids quantified by LC-MS/MS. Data are shown in a matrix format: each row represents a single phospholipid, and each column represents the lipid extract of the brain of one animal (6 mice and 7 naked mole-rat). Each colour patch represents the normalized quantity of phospholipid (row) in a single animal brain (column), with a continuum of quantity from bright green (lowest) to bright red (highest). The pattern and length of the branches in the left dendrogram reflect the relatedness of the phospholipids. The dashed red line is the dendrogram distance used to cluster the phospholipids. The pattern and length of the branches in the top dendrogram reflect the relatedness of the different animals. (F) Heat map of the different sphingomyelins and ceramides quantified by LC-MS/MS. Data are shown in a matrix format: each row represents a single sphingomyelin or ceramide, and each column represents the lipid extract of the brain of one animal (6 mice and 7 naked mole-rat). Each colour patch represents the normalized quantity of sphingomyelin or ceramide (row) in a single animal brain (column), with a continuum of quantity from bright green (lowest) to bright red (highest). The pattern and length of the branches in the left dendrogram reflect the relatedness of the sphingomyelins and ceramides. The dashed red line is the dendrogram distance used to cluster the phospholipids. The pattern and length of the branches in the top dendrogram reflect the relatedness of the different animals.