Figure 6. AFM results from mouse samples (n=1220) and naked mole-rat samples (n=2859) lipid bilayer indentations. (A, B) Example indentation on a naked mole-rat bilayer (A), where multiple breakthrough events were detected only the event closest to the mica (B) was recorded. The points indicated by the dashed and solid arrow in panel B show the start and end of the breakthrough event respectively. Thickness was recorded as the difference in separation between the points and breakthrough force was recorded at the start of the breakthrough indicated by the dashed arrow. (C) Thickness values of 3.1 ± 0.4 nm and 2.8 ± 0.6 nm were recorded for the naked mole-rat and mouse lipid bilayers respectively. (D) Breakthrough force values of 8.1 ± 0.4 nN and 1.2 ± 0.2 nN were recorded for the naked mole-rat and mouse lipid bilayers respectively. Data are expressed as mean ± SEM. Statistical analysis was performed using the unpaired t test. *p<0.05, significantly different from mouse group.