Figure 4. Phenotypic screening of the anti-CSC activity of oleacein mimetics (I).Left. Comparative analysis of IC50 values of the computationally-predicted oleacein mimetics in 2D monolayer cultures and 3D mammosphere systems. With 10 μmol/L as a cutoff, 4/16 compounds tested were more potent in 3D than in 2D and were selected as anti-CSC candidates; 1/16 compounds tested was equally potent in 3D and in 2D and was designated as cytotoxic. Right. CHEMBL structures of the computationally-predicted oleacein mimetics with anti-CSC (blue box) and cytotoxic (red box) activity.