Research Paper Volume 13, Issue 1 pp 460—476

Dihydromyricetin promotes longevity and activates the transcription factors FOXO and AOP in Drosophila

DHM can improve stress tolerance and delay the intestinal dysfunction. (A) Survival curve of fly starvation on the 1.5% agar food, showing DHM improves starvation resistance (p=2.7x10-5, log-rank test). (B) TAG levels of wDah flies that were measured after 14 days of DHM treatment. (C) Oil red O staining of 14 days old fly guts. (D) Drosophila climbing ability from 10 days to 35 days (ns means “No significant difference”, * means ppE) The percentage of ‘‘Smurf’’ flies. DHM treatment improves intestinal integrity in aged flies. (***p 90 females for each genotype). (F) The numbers of colony-forming units (CFUs) in intestinal extracts of control and DHM-treated flies (ns means “No significant difference”, * means ppLactobacilli, ACE: Acetobacteria, ENT: Enterobacteria.

Figure 2. DHM can improve stress tolerance and delay the intestinal dysfunction. (A) Survival curve of fly starvation on the 1.5% agar food, showing DHM improves starvation resistance (p=2.7x10-5, log-rank test). (B) TAG levels of wDah flies that were measured after 14 days of DHM treatment. (C) Oil red O staining of 14 days old fly guts. (D) Drosophila climbing ability from 10 days to 35 days (ns means “No significant difference”, * means p<0.05, **means p<0.01). (E) The percentage of ‘‘Smurf’’ flies. DHM treatment improves intestinal integrity in aged flies. (***p < 0.001, n >90 females for each genotype). (F) The numbers of colony-forming units (CFUs) in intestinal extracts of control and DHM-treated flies (ns means “No significant difference”, * means p<0.05, **means p<0.01; n=10; repeat=3), NR: Nutrient-rich medium, LMRS: Lactobacilli, ACE: Acetobacteria, ENT: Enterobacteria.