Figure 3. DHM promotes FOXO activity. (A) The protein expression levels of pAKT, AKT by Western blots. The bar graph illustrated the densitometry of the blots (p=0.031, n=2, repeat=3). (B) dFOXO antibody staining in the guts. The nuclei are stained with DAPI in blue, the white box shows the zoom of the corresponding position. The intensity quantifications of FOXO/Area of DAPI are shown as bar plots (n=7, 6 p=0.0072, t-test and the data are presented as the mean ± SEM). (C) The FOXO target genes 4ebp and inr expression levels (relative to actin-5C) in the DHM-treated flies and control (p=0.012, p=0.002; n=3). (D) Knockdown of foxo in the adult fly shortens lifespan. Addition of the DHM can slightly rescue the lifespan of foxo knockdown flies.