Research Paper Volume 13, Issue 1 pp 460—476

Dihydromyricetin promotes longevity and activates the transcription factors FOXO and AOP in Drosophila

DHM promotes FOXO activity. (A) The protein expression levels of pAKT, AKT by Western blots. The bar graph illustrated the densitometry of the blots (p=0.031, n=2, repeat=3). (B) dFOXO antibody staining in the guts. The nuclei are stained with DAPI in blue, the white box shows the zoom of the corresponding position. The intensity quantifications of FOXO/Area of DAPI are shown as bar plots (n=7, 6 p=0.0072, t-test and the data are presented as the mean ± SEM). (C) The FOXO target genes 4ebp and inr expression levels (relative to actin-5C) in the DHM-treated flies and control (p=0.012, p=0.002; n=3). (D) Knockdown of foxo in the adult fly shortens lifespan. Addition of the DHM can slightly rescue the lifespan of foxo knockdown flies.

Figure 3. DHM promotes FOXO activity. (A) The protein expression levels of pAKT, AKT by Western blots. The bar graph illustrated the densitometry of the blots (p=0.031, n=2, repeat=3). (B) dFOXO antibody staining in the guts. The nuclei are stained with DAPI in blue, the white box shows the zoom of the corresponding position. The intensity quantifications of FOXO/Area of DAPI are shown as bar plots (n=7, 6 p=0.0072, t-test and the data are presented as the mean ± SEM). (C) The FOXO target genes 4ebp and inr expression levels (relative to actin-5C) in the DHM-treated flies and control (p=0.012, p=0.002; n=3). (D) Knockdown of foxo in the adult fly shortens lifespan. Addition of the DHM can slightly rescue the lifespan of foxo knockdown flies.