Research Paper Volume 13, Issue 2 pp 1947—1961

Erythrocyte membrane protein band 4.1-like 3 inhibits osteosarcoma cell invasion through regulation of Snai1-induced epithelial-to-mesenchymal transition

EPB41L3 knockdown decreases osteosarcoma cell viability. (A) Knockdown efficiency of EPB41L3 siRNAs (siEPB41L3#1 and siEPB41L3#2) versus control siRNA (siNC) was examined by qRT-PCR. Data were presented as the mean ± standard error of the mean of two independent experiments. (B) U2OS and MG63 were transfected with siEPB41L3 (siEPB41L3#1 or siEPB41L3#2) or control siRNA (siNC), and the cell viability was determined by CCK-8 assay assessed at 96 h post-transfection. Data were presented as the mean ± SD of three experimental repeats. (C) Colony formation assays for the effects of EPB41L3 silence in U2OS and MG63. Statistical results of colony formation numbers normalized to siNC were presented. (D) The efficiency of knocking down by lentiviral delivery of shRNA (shEPB41L3) was assayed by qRT-PCR (left panel) and western blot (right panel). (E) CCK-8 assays for the effects of stably expressing shNC and shEPB41L3 on the proliferation of U2OS and MG63 cells. (F) Colony formation assays for the effects of stably expressing shNC and shEPB41L3 in U2OS and MG63. Statistical results of colony formation numbers normalized to shNC were presented. NC, negative control; si, small interfering RNA.

Figure 2. EPB41L3 knockdown decreases osteosarcoma cell viability. (A) Knockdown efficiency of EPB41L3 siRNAs (siEPB41L3#1 and siEPB41L3#2) versus control siRNA (siNC) was examined by qRT-PCR. Data were presented as the mean ± standard error of the mean of two independent experiments. (B) U2OS and MG63 were transfected with siEPB41L3 (siEPB41L3#1 or siEPB41L3#2) or control siRNA (siNC), and the cell viability was determined by CCK-8 assay assessed at 96 h post-transfection. Data were presented as the mean ± SD of three experimental repeats. (C) Colony formation assays for the effects of EPB41L3 silence in U2OS and MG63. Statistical results of colony formation numbers normalized to siNC were presented. (D) The efficiency of knocking down by lentiviral delivery of shRNA (shEPB41L3) was assayed by qRT-PCR (left panel) and western blot (right panel). (E) CCK-8 assays for the effects of stably expressing shNC and shEPB41L3 on the proliferation of U2OS and MG63 cells. (F) Colony formation assays for the effects of stably expressing shNC and shEPB41L3 in U2OS and MG63. Statistical results of colony formation numbers normalized to shNC were presented. NC, negative control; si, small interfering RNA.