Figure 1. NC regulates cell viability and apoptosis. (A) The Effects of NC on the viability of lung cancer cells for 72 h were measured using the MTT assay. *P<0.05, **P<0.01 vs the control. (B) Cell apoptotic death in lung cancer cells with NC treatment at 72 h was tested by the Annexin V-FITC/PI approach. (C) Early and late apoptosis rates were presented for panel B. (D) Early NEDD4 mRNA level was detected by RT-PCR in cells with NC treatment for 72 h. (E) Left panel: NEDD4 levels were examined by western blotting in lung cancer cells with NC exposure for 72 h. Right panel: Quantitative data are presented for NEDD4 levels. *P<0.05,**P<0.01, vs the control.