Research Paper Volume 13, Issue 1 pp 782—793

Nitidine chloride suppresses NEDD4 expression in lung cancer cells

NEDD4 overexpression rescues NC-mediated cell growth suppression and apoptosis. (A) Left panel: NEDD4 expression was tested by immunoblotting in lung cancer cells with NEDD4 plasmid transfection plus NC treatment. Right panel: Quantitative data are shown for NEDD4 levels. **P##pB) The MTT assay was carried out to test the viability of lung cancer cells after NEDD4 overexpression and NC treatment. (C) Apoptosis of lung cancer cells was examined by flow cytometry after the combination treatment. (D). Apoptosis rates were presented for panel C.

Figure 3. NEDD4 overexpression rescues NC-mediated cell growth suppression and apoptosis. (A) Left panel: NEDD4 expression was tested by immunoblotting in lung cancer cells with NEDD4 plasmid transfection plus NC treatment. Right panel: Quantitative data are shown for NEDD4 levels. **P<0.01, vs the control; ##p<0.01, vs NC only or NEDD4 plasmid transfection only. CTR: control; NC: Nitidine chloride; cDNA: NEDD4 cDNA vector; Both: NEDD4 cDNA plus NC. (B) The MTT assay was carried out to test the viability of lung cancer cells after NEDD4 overexpression and NC treatment. (C) Apoptosis of lung cancer cells was examined by flow cytometry after the combination treatment. (D). Apoptosis rates were presented for panel C.