Research Paper Advance Articles pp 23497—23508

Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice

Figure 1. Blockade of TNFα by Etanercept inhibited sarcopenia during spontaneous aging. Measurement of cross-sectional area (CSA) (A) and fiber size distribution (B) in GS of 12 months old mice and in control (saline) or Etanercept-treated mice at 22 and 28 months of age. *=p<0.05, **= p<0.01 and *** =p<0.001. (C) Representative histochemical images of GS muscle sections analyzed in 12 months old mice and in control or Etanercept-treated mice at 22 and 28 months of age. Scale bar=50 μm (magnification of the16x) (D). MRI measurement of muscle volume for control or Etanercept-treated mice at 12, 19 and 22 months of age. *=p<0.05 in comparison to 12 months of age (E) Body weight of control and Etanercept-treated mice monitored between 16 and 28 months of age. ** =p<0.01 vs 16 months of age, °= p<0.05 vs weight of control mice measured at the same time point. Data are show as mean ± standard error.