Research Paper Advance Articles pp 23497—23508

Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice

Figure 3. TNFα blockade inhibited the loss of type II fast twitch fibers. (A) Percentage of Type II (left), Type IIB (middle) and Type I (right) fibers in GS muscles of 12 months old mice in controls (saline) or Etanercept-treated mice at 22 and 28 months of age, *=p<0.05, **= p<0.01 and ***=p<0.001. (B) Representative images of GS muscle sections. Type I fibers are blue, type IIA are green, type IIB are red. Scale bar=50 μm. (C) Percentage of Type II (left), Type IIB (middle) and Type I (right) fibers in soleus muscles of 12 months old mice or in control or Etanercept-treated mice at 22 and 28 months of age. *=p<0.05, **= p<0.01. (D) Representative images of soleus muscle sections. Scale bar=50 μm.