Research Paper Volume 12, Issue 23 pp 24301—24317

Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling

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Figure 5.

CSE suppresses PI3K/AKT cascade in epithelial cells. (A, B) BEAS-2B cells were treated with CSE at various time points as indicated. Cells lysates were analyzed with mTOR, PI3Kp110, PDK1, INPP4b, PI3Kp85, PTEN, PPP2AC, PRMT6, and GAPDH immunoblotting. The densitometry of the blots was presented in the lower panel. (C, D) HSAECs cells were treated with CSE at a course of time as indicated. Cells lysates were analyzed with mTOR, PI3Kp110, PDK1, INPP4b, PI3Kp85, PTEN, PPP2AC, PRMT6, and GAPDH immunoblotting. The densitometric results were plotted in the lower panels. (E, F) HSAECs cells were exposed to room air or cigarette smoke. Cells lysates were collected and analyzed with indicated antibodies immunoblotting. RA: room air; CS: cigarette smoke. The densitometric blots were showed in the lower panel of each figure. Results were representative of n=3 experiments. Statistical significance was indicated as *: p < .05.