Research Paper Volume 13, Issue 2 pp 2118—2134

Figure 3. IGF2 ameliorates the meiotic defects of aged mouse oocytes. (A) Representative images of spindle/chromosome organization in control and IGF2-treated oocytes from aged mice. Spindles were stained with an antibody against α-tubulin (green), and chromosomes were counter-stained with Hoechst 33342 (blue). Scale bar = 30 μm. (B) Quantification of abnormal spindle/chromosomes oocytes in control (n = 95) and IGF2-treated (n =105) oocytes groups. A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (C) Representative images of CM-H2DCFDA fluorescence (green) in control and IGF2-treated oocytes. Scale bar = 20 μm. (D) Quantification of ROS signals in control oocytes (n = 25) and IGF2-treated oocytes (n = 21). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (E) Adenosine triphosphate (ATP) contents in control oocytes (n = 50) and IGF2-treated oocytes (n = 50). A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM.