Research Paper Volume 13, Issue 2 pp 2118—2134

IGF2 improves the developmental competency and meiotic structure of oocytes from aged mice

IGF2 improves the mitochondrial ultrastructure of oocytes from aged mice. (A) Representative TEM micrographs of mitochondria from control and IGF2-treated oocytes at 2,500x magnification. Scale bar = 1 μm. Note the normal (Mn) and vacuolated (Mv) mitochondria. (B) Quantification of mitochondria per defined region of interest (ROI) in control and IGF2-treated oocytes. n=9 oocytes for each group. A Student’s t-test (two-tailed). *p C) Representative TEM micrographs of mitochondria from control and IGF2-treated oocytes at 60,000x magnification. Inner membrane (IM), outer membrane (OM), and intermembrane space (IMS). Scale bar = 200 nm.

Figure 5. IGF2 improves the mitochondrial ultrastructure of oocytes from aged mice. (A) Representative TEM micrographs of mitochondria from control and IGF2-treated oocytes at 2,500x magnification. Scale bar = 1 μm. Note the normal (Mn) and vacuolated (Mv) mitochondria. (B) Quantification of mitochondria per defined region of interest (ROI) in control and IGF2-treated oocytes. n=9 oocytes for each group. A Student’s t-test (two-tailed). *p < 0.05. Error bars indicate the SEM. (C) Representative TEM micrographs of mitochondria from control and IGF2-treated oocytes at 60,000x magnification. Inner membrane (IM), outer membrane (OM), and intermembrane space (IMS). Scale bar = 200 nm.