Research Paper Volume 13, Issue 2 pp 2198—2211

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

MiR-198 mediated the regulatory effects of TRIM14 on the progression of TMZ-resistant glioma cells. (A) Western Blotting analysis was used to measure TRIM14 expression in U138/TMZ and LN18/TMZ cells after co-transfected with miR-198 mimic and TRIM14 overexpression plasmid (pc-TRIM14). (B, C) The viability of U138/TMZ and LN18/TMZ cells co-transfected with miR-198 mimic and pc-TRIM14 was detected by CCK-8 and colony formation assays. (D) Apoptotic rate was detected by flow cytometry. (E) The TMZ resistance of cells was measured by CCK-8 assay. *P

Figure 8. MiR-198 mediated the regulatory effects of TRIM14 on the progression of TMZ-resistant glioma cells. (A) Western Blotting analysis was used to measure TRIM14 expression in U138/TMZ and LN18/TMZ cells after co-transfected with miR-198 mimic and TRIM14 overexpression plasmid (pc-TRIM14). (B, C) The viability of U138/TMZ and LN18/TMZ cells co-transfected with miR-198 mimic and pc-TRIM14 was detected by CCK-8 and colony formation assays. (D) Apoptotic rate was detected by flow cytometry. (E) The TMZ resistance of cells was measured by CCK-8 assay. *P < 0.05, **P < 0.01, ***P < 0.001.