Figure 6. Expression of mature myocardial markers on mRNA level. (A–C) The relative mRNA expression of mature myocardial markers cTnT, cTnI and α-actin on different matrix of 13-16 kPa, 62-68 kPa and control group at the same time point, the 1st day, the 7th day, and the 14th day. On the 7th day and the 14th day, the expression of cTnI and α-actin on 13-16 kPa matrix were the highest. The cTnT on 13-16 kPa was expressed the most on the 7th day, but its expression decreased on the 14th day. (D, E) The expression of the three mature myocardial markers on the same stiffness at different time points. The expression of cTnI and α-actin increased over time on 13-16 kPa matrix, and the expression of cTnT rose to the highest on the 7th day but decreased on the 14th day. On the 62-68 kPa matrix, the expression of α-actin increased over time, while the expression of cTnT and cTnI in the matrix stiffness groups were lower than that of the control group. *P<0.05, **P<0.01, ***P<0.001. n=3.