Research Paper Volume 13, Issue 2 pp 2231—2250

Matrix stiffness regulates myocardial differentiation of human umbilical cord mesenchymal stem cells

The effect of matrix stiffness on the expression of Piezo1. (A) QRT-PCR detection of Piezo1 on different matrix of 13-16 kPa, 62-68 kPa and control group at the same time point, the 1st day, the 7th day and the 14th day. On the 1st day and the 7th day, Piezo1 increased with increasing matrix stiffness, and on the 14th day, the expression of Piezo1 on matrix of 13-16 kPa was significantly higher than that of the other two groups. (B) The expression tendency of Piezo1 on the same stiffness changed with time. On the same stiffness, Piezo1 was highly expressed only on the first day. (C, D) Immunofluorescence detection in the same condition as qRT-PCR. The results also showed that compared with other time points, Piezo1 was expressed the most on the 1st day, and compared with other stiffness, it was expressed most on 62-68 kPa. n=3.

Figure 9. The effect of matrix stiffness on the expression of Piezo1. (A) QRT-PCR detection of Piezo1 on different matrix of 13-16 kPa, 62-68 kPa and control group at the same time point, the 1st day, the 7th day and the 14th day. On the 1st day and the 7th day, Piezo1 increased with increasing matrix stiffness, and on the 14th day, the expression of Piezo1 on matrix of 13-16 kPa was significantly higher than that of the other two groups. (B) The expression tendency of Piezo1 on the same stiffness changed with time. On the same stiffness, Piezo1 was highly expressed only on the first day. (C, D) Immunofluorescence detection in the same condition as qRT-PCR. The results also showed that compared with other time points, Piezo1 was expressed the most on the 1st day, and compared with other stiffness, it was expressed most on 62-68 kPa. n=3.