Figure 4. ARRB2 deficiency inhibits PI3K/Akt signaling activation. (A) PI3K, p-PI3K, AKT, p-AKT protein levels in liver tissue detected by Western Blotting in Sham, WT + IR and KO + IR groups. (B) Quantitative analysis of p-PI3K/PI3K levels and p-Akt/Akt levels. (C) and (D), Immunohistochemical staining of p-Akt in liver tissues (×200). The data are presented as the Mean ± SD, n = 6. * P< 0.01, compared to Sham group, #P < 0.01, compared to WT + IR group by Student’s-t test.