Research Paper Advance Articles pp 24057—24080

Age-related DNA methylation changes are sex-specific: a comprehensive assessment

Figure 2. Validation of FIGN and PRR4 loci by EpiTYPER. (A) Methylation of CpG unit 9 in FIGN amplicon vs age. (B) Methylation of CpG unit 3 in PRR4 amplicon vs age. (A and B) For each CpG unit, DNA methylation in controls (general population), centenarians, centenarian's offspring and persons with Down syndrome is reported vs the age of the subjects. Males are in blue, females are in red. Linear regression between DNA methylation and age was calculated separately for males and females in control subjects and was reported in each plot. (C) Boxplots of DNA methylation of CpG unit 9 in FIGN amplicon in aged controls and centenarians. (D) Boxplots of DNA methylation of CpG unit 3 in PRR4 amplicon in healthy controls and persons with Down syndrome.