Figure 7. The knockdown of B3GNT3 suppresses the proliferation, invasion, and EMT of PC cells. (A) RT-PCR analysis validated the knockdown of B3GNT3 in PANC-1 and AsPC-1 cells transfected with sh-B3GNT3. (B) Assessment of cell proliferation using the MTT assay. (C) Transwell assay was performed to determine the invasive capacity of PANC-1 and AsPC-1 cells transfected with sh-B3GNT3. (D) WB analysis to investigate the association between B3GNT3 and EMT in PC. PC: pancreatic cancer; EMT: epithelia-mesenchymal transition; WB: western blot. (*p<0.05; **p<0.01;***p<0.001; ****p<0.0001).