Research Paper Volume 13, Issue 2 pp 2348—2364

Upregulation of HOTAIRM1 increases migration and invasion by glioblastoma cells

SNAI2 suppress CDH1 to induce HOTAIRM1 in GBM cells. (A) The potential CDH1-binding sites (BS3) in the HOTAIRM1 promoter region was predicted using the high-quality transcription factor binding profile database (JASPAR). (B) The predicted region was transcriptionally responsive to CDH1 overexpression, as shown in luciferase reporter assays. (C) CDH1 bound to both predicted binding sites in HOTAIRM1 promoter, as shown in ChIP assays. (D) Relative expression of HOTAIRM1 in GBM cells transfected with sh-ctrl or sh-CDH1. (E) Relative expression of HOTAIRM1 in GBM cells transfected with vector or CDH1. (F) Relative expression of HOTAIRM1 in GBM cells transfected with vector or CDH1 or co-transfected with CDH1 and SNAI2. (G) Relative expression of HOTAIRM1 in GBM cells transfected with sh-ctrl or sh-CDH1 or co-transfected with sh-CDH1 and sh-SNAI2.

Figure 6. SNAI2 suppress CDH1 to induce HOTAIRM1 in GBM cells. (A) The potential CDH1-binding sites (BS3) in the HOTAIRM1 promoter region was predicted using the high-quality transcription factor binding profile database (JASPAR). (B) The predicted region was transcriptionally responsive to CDH1 overexpression, as shown in luciferase reporter assays. (C) CDH1 bound to both predicted binding sites in HOTAIRM1 promoter, as shown in ChIP assays. (D) Relative expression of HOTAIRM1 in GBM cells transfected with sh-ctrl or sh-CDH1. (E) Relative expression of HOTAIRM1 in GBM cells transfected with vector or CDH1. (F) Relative expression of HOTAIRM1 in GBM cells transfected with vector or CDH1 or co-transfected with CDH1 and SNAI2. (G) Relative expression of HOTAIRM1 in GBM cells transfected with sh-ctrl or sh-CDH1 or co-transfected with sh-CDH1 and sh-SNAI2.