Research Paper Volume 13, Issue 2 pp 2348—2364

Upregulation of HOTAIRM1 increases migration and invasion by glioblastoma cells

SNAI2 promotes migration and invasion of GBM cells and tumor growth in vivo. (A) Wound healing assays were used to analyze migration of GBM cells. (B) Matrigel invasion assays were used to analyze invasion of GBM cells. (C) Morphological changes of GBM cells were imaged to analyze EMT process of GBM cells. (D) EMT-associated proteins in GBM cells were determined by western blotting. (E) Representative images of subcutaneous tumors originated from transfected pGBM1 cells on the days indicated. (F) Growth curve of tumors originated from transfected pGBM1 cells. (G) Weight of tumors originated from transfected pGBM1 cells. (H) Relative expression of miR-153-5p in tumors originated from transfected pGBM1 cells. (I) Schematic illustration of proposed model depicting important role of the lncRNA HOTAIRM1 in regulating cell migration and invasion. *P P

Figure 7. SNAI2 promotes migration and invasion of GBM cells and tumor growth in vivo. (A) Wound healing assays were used to analyze migration of GBM cells. (B) Matrigel invasion assays were used to analyze invasion of GBM cells. (C) Morphological changes of GBM cells were imaged to analyze EMT process of GBM cells. (D) EMT-associated proteins in GBM cells were determined by western blotting. (E) Representative images of subcutaneous tumors originated from transfected pGBM1 cells on the days indicated. (F) Growth curve of tumors originated from transfected pGBM1 cells. (G) Weight of tumors originated from transfected pGBM1 cells. (H) Relative expression of miR-153-5p in tumors originated from transfected pGBM1 cells. (I) Schematic illustration of proposed model depicting important role of the lncRNA HOTAIRM1 in regulating cell migration and invasion. *P < 0.05, **P < 0.01.