Research Paper Volume 13, Issue 2 pp 2506—2518

Silencing of lncRNA MIAT alleviates LPS-induced pneumonia via regulating miR-147a/NKAP/NF-κB axis

Knockdown of MIAT alleviates LPS-induced inflammation and injury in mice. LV-sh-MIAT or LV-sh-NC was intratracheally injected into mice. (A) The knockdown efficiency of sh-MIAT was determined by qRT-PCR. (B) Survival plots for mice in different groups. (C) H&E staining for lung sections in different groups. Scale bar, 60 μm. (D) Wet dry mass ratio (W/D) of lungs was calculated. (E, F) macrophages and neutrophils in alveolar lavage fluid was collected and calculated. (G) Myeloperoxidase (MPO) of lungs was examined. (H) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (I) qRT-PCR analysis for E-cadherin and Sftpc expression. Data are mean ± SD; *P

Figure 2. Knockdown of MIAT alleviates LPS-induced inflammation and injury in mice. LV-sh-MIAT or LV-sh-NC was intratracheally injected into mice. (A) The knockdown efficiency of sh-MIAT was determined by qRT-PCR. (B) Survival plots for mice in different groups. (C) H&E staining for lung sections in different groups. Scale bar, 60 μm. (D) Wet dry mass ratio (W/D) of lungs was calculated. (E, F) macrophages and neutrophils in alveolar lavage fluid was collected and calculated. (G) Myeloperoxidase (MPO) of lungs was examined. (H) qRT-PCR analysis for IL 1β, IL 6 and TNFα expression. (I) qRT-PCR analysis for E-cadherin and Sftpc expression. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.