Research Paper Volume 13, Issue 2 pp 2575—2592

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial–mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway

The effect of β-elemene and RT on the expression of Prx-1/NF-kB /iNOS pathway. A549 cells were treated with single RT (4 Gy) or β-elemene prior to RT (4 Gy) Cell lysates were extracted 12 h after RT. (A) The expression of p-IkBα, p-IKK α/β, and p-p65 in cytoplasm were measured by Western blot. (B) The cytoplasmic and nuclear proteins were separated, and the expression of p50/p65 in the nucleus was measured by Western blot. (C) The subcellular localization of p50, p65 in A549 cells treated with β-elemene or RT alone or their combination was examined by confocal microscopy. More than 100 cells were inspected per experiment, and the cells with typical morphology were presented. (D) The A549 cells were treated with β-elemene or RT alone or their combination. The expression of Prx-1 and iNOS at mRNA and protein levels were measured by RT-RCR and Western blot.

Figure 6. The effect of β-elemene and RT on the expression of Prx-1/NF-kB /iNOS pathway. A549 cells were treated with single RT (4 Gy) or β-elemene prior to RT (4 Gy) Cell lysates were extracted 12 h after RT. (A) The expression of p-IkBα, p-IKK α/β, and p-p65 in cytoplasm were measured by Western blot. (B) The cytoplasmic and nuclear proteins were separated, and the expression of p50/p65 in the nucleus was measured by Western blot. (C) The subcellular localization of p50, p65 in A549 cells treated with β-elemene or RT alone or their combination was examined by confocal microscopy. More than 100 cells were inspected per experiment, and the cells with typical morphology were presented. (D) The A549 cells were treated with β-elemene or RT alone or their combination. The expression of Prx-1 and iNOS at mRNA and protein levels were measured by RT-RCR and Western blot.