Research Paper Advance Articles pp 24394—24423

E3 ligase FBXW7 restricts M2-like tumor-associated macrophage polarization by targeting c-Myc

Figure 3. FBXW7 expression decreases in M2-like TAMs. (A, B) Peritoneal macrophages were stimulated with conditioned medium containing LLC supernatant for the indicated time periods. The mRNA (A) and protein (B) expression levels of Arg1, Ym1, and FBXW7 were measured by qRT-PCR and immunoblotting, respectively. (C, D) BMDMs were incubated in conditioned medium, and the mRNA (C) and protein (D) expression levels of Arg1, Ym1, and FBXW7 were measured by qRT-PCR and immunoblotting. Data are shown as the mean ± SD and are representative of three independent experiments. n=3. **P < 0.01; ***P < 0.001; ****P < 0.0001 (one-way ANOVA (A, C)).