Research Paper Advance Articles pp 24424—24440

FOXM1/DVL2/Snail axis drives metastasis and chemoresistance of colorectal cancer

Figure 3. FOXM1 triggered EMT-like molecular changes and P-gp expression via up-regulating Snail. Western blotting for expressions of Snail and FOXM1 in HCT-8 cells transfected with pcDNA3.1 or pcDNA3.1-FOXM1 for 72 h (A), HCT-8/L-OHP and HCT-8/VCR cells transfected with shNC or shFOXM1 for 72 h (B, C). The expressions of E-cadherin, N-cadherin, Vimentin, P-gp, Snail and FOXM1 in HCT-8 cells transfected with pcDNA3.1, pcDNA3.1-FOXM1, or pcDNA3.1-FOXM1 plus shSnail for 72 h (D), HCT-8/L-OHP and HCT-8/VCR cells transfected with shNC, shFOXM1, or shFOXM1 plus pcDNA3.1-Snail (E, F). In each case, the blot is representative of immunoblots resulting from three separate experiments.