Research Paper Advance Articles pp 24424—24440

FOXM1/DVL2/Snail axis drives metastasis and chemoresistance of colorectal cancer

Figure 4. FOXM1 induced metastasis and chemoresistance of CRC via Snail. Transwell and matrigel invasion assays for migratory and invasive behaviors (A), and MTT assay for oxaliplatin (B, C) and vincristine (D, E) sensitivity and IC50 in HCT-8 cells transfected with pcDNA3.1, pcDNA3.1-FOXM1, or pcDNA3.1-FOXM1 plus shSnail for 72 h, as indicated. Transwell and matrigel invasion assays for migratory and invasive behaviors (F, G), and MTT assay for oxaliplatin (H, I) or vincristine (J, K) sensitivity and IC50 in HCT-8/L-OHP or HCT-8/VCR cells transfected with shNC, shFOXM1, or shFOXM1 plus pcDNA3.1-Snail for 72 h, as indicated. Data are expressed as mean ± SD of three independent experiments. *P < 0.05.