Figure 2. Protective efficacy of neuritin overexpression revealed by upregulation of neurite markers. (A) Western blots of Neuritin and NF-200 expression level at the indicated time points after TGI. β-actin was used as the gel loading control. Tg-TGI, neuritin transgenic mice subjected to TGI; WT-TGI, WT mice subjected to TGI. (B, C) Quantitative analysis of neuritin and NF-200 expression shown in (A) respectively. (D) Immunohistochemical analysis of Neuritin expression in the hippocampal CA1 region at the indicated time points after TGI. Small black rectangular frame/large black rectangular frame=1/4. (E) Quantitative analysis of Neuritin expression data (OD value ratio) shown in (D). (F) Immunohistochemical analysis of NF-200 expression in the hippocampal CA1 region at the indicated time points after TGI. (G) Quantitative analysis of NF-200 expression data (OD value ratio) shown in (F). Data expressed as mean ± S.E.M. (n = 6). scale bar=50 μm, *p < 0.05, **p < 0.01 and ***p < 0.001 vs. WT-TGI group.