Research Paper Volume 13, Issue 1 pp 1357—1368

miR-18a increases insulin sensitivity by inhibiting PTEN

Overexpression of miR-18a enhances glucose metabolism and insulin sensitivity in male RL-18a mice. (A) MiR-18a transgene levels in multiple tissues from RL-18a mice were determined using qRT-PCR. (B) Body weights of RL-18a and control mice (n=7). (C) Blood glucose concentrations in fed and 12-hour-fasted mice at different times. (D) Serum insulin concentrations in fed and 12-hour-fasted mice. (E, F) Glucose tolerance test results determined with an enzyme-linked immunosorbent assay in 12-hour-fasted mice (E), and the area under the curve (AUC) for this test (F). (G, H) Insulin tolerance test in 12-hour-fasted mice (G), and the AUC for this test (H). (I) PTEN expression in skeletal muscle (SK), adipose tissue (AT) and liver samples from RL-18a mice, assessed using qRT-PCR. (J) PTEN expression in SK, AT and liver samples from RL-18a mice, assessed using Western blotting. n=7 male mice/group.

Figure 2. Overexpression of miR-18a enhances glucose metabolism and insulin sensitivity in male RL-18a mice. (A) MiR-18a transgene levels in multiple tissues from RL-18a mice were determined using qRT-PCR. (B) Body weights of RL-18a and control mice (n=7). (C) Blood glucose concentrations in fed and 12-hour-fasted mice at different times. (D) Serum insulin concentrations in fed and 12-hour-fasted mice. (E, F) Glucose tolerance test results determined with an enzyme-linked immunosorbent assay in 12-hour-fasted mice (E), and the area under the curve (AUC) for this test (F). (G, H) Insulin tolerance test in 12-hour-fasted mice (G), and the AUC for this test (H). (I) PTEN expression in skeletal muscle (SK), adipose tissue (AT) and liver samples from RL-18a mice, assessed using qRT-PCR. (J) PTEN expression in SK, AT and liver samples from RL-18a mice, assessed using Western blotting. n=7 male mice/group.