Figure 4. PTEN is a target gene of miR-18a. (A) The predicted binding sequence for miR-18a within the human PTEN 3’UTR. Seed sequences are highlighted. (B) Luciferase reporter assay in 3T3-L1 cells. The bar graph displays the mean ± SD of three independent transfection experiments. *P<0.05. (C, D) PTEN mRNA levels in 3T3-L1 and C2C12 cells transfected with LV-miR-18a, anti-miR-18a or the corresponding controls (LV-con and anti-con, respectively). (E, F) PTEN protein levels were assessed using Western blotting in 3T3-L1 and C2C12 cells transfected with LV-miR-18a, LV-con, anti-miR-18a or anti-con.