Research Paper Volume 13, Issue 1 pp 1357—1368

miR-18a increases insulin sensitivity by inhibiting PTEN

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Figure 5. Functional recovery test confirming that PTEN overexpression reverses miR-18a-induced AKT phosphorylation. Western blot analysis of insulin-stimulated AKT phosphorylation in 3T3-L1 and C2C12 cells transfected with different plasmids.