Research Paper Volume 13, Issue 2 pp 2700—2726

Identification of the ceRNA networks in α-MSH-induced melanogenesis of melanocytes

Confirmation of the quaternary ceRNA network regulating TYR. (A) After transfection with siRNAs of ENST00000606533 in MCs for 48 h, the transfection efficiency of si-ENST00000606533 mix (mixing the three siRNAs) was most significant, and the expression of circ

Figure 5. Confirmation of the quaternary ceRNA network regulating TYR. (A) After transfection with siRNAs of ENST00000606533 in MCs for 48 h, the transfection efficiency of si-ENST00000606533 mix (mixing the three siRNAs) was most significant, and the expression of circ_0091223, miR-1291, and TYR were detected by transfection with si-ENST00000606533 mix. (B) After transfection with siRNAs of circ_0091223 for 48 h, the transfection efficiency of si-circ_0091223 2 was most significant, and the expression of ENST00000606533, miR-1291, and TYR were detected by transfection with si-circ_0091223 2. (C) After transfection with miR-1291 mimics for 48 h, the transfection efficiency, the expression of ENST00000606533, circ_0091223, and TYR were detected. (D) After treated with α-MSH, the MCs were transfected with si-ENST00000606533 mix, or si-circ_0091223 2, or miR-1291 mimics, and the expression of ENST00000606533, circ_0091223, miR-1291, and TYR were detected. (E) After co-transfection with siRNAs of ENST00000606533 and miRNA_1291 inhibitor for 48 h, the expression of ENST00000606533, circ_0091223, miR-1291, and TYR were detected. (F) After co-transfection with siRNA of circ_0091223 and miR-1291 inhibitor for 48 h, the expression of ENST00000606533, circ_0091223, miR-1291, and TYR were detected. (G) After treated with α-MSH and/or si-ENST00000606533 mix, or si-circ_0091223 2, or miR-1291 mimics, the protein level of TYR were detected. (H) After treated with α-MSH and/or si-ENST00000606533 mix, or si-circ_0091223 2, or miR-1291 mimics, the Masson-Fontana melanin staining were detected.