Research Paper Volume 13, Issue 2 pp 2750—2767

ALDH2 protects naturally aged mouse retina via inhibiting oxidative stress-related apoptosis and enhancing unfolded protein response in endoplasmic reticulum

The expression of apoptosis related proteins in aged ALDH2 overexpression and aged Alda1-treated mice. (A) A typical WB image of CHOP, caspase9 and caspase12 in aged ALDH2 overexpression mice; (B–D) The expression of CHOP, caspase9 and caspase12 in aged ALDH2 overexpression mice; (E) A typical WB picture of CHOP, caspase9 and caspase12 in aged Alda1-treated mice; (F–H) The expression of CHOP, caspase9 and caspase12 in aged Alda1-treated mice. All analyses were performed in duplicate. Values are presented as the mean ± SD, n = 4 mice per group. *Pvs young (WT) or Aged (DMSO) and aged (Alda1) vs young; **Pvs young (WT) or Aged (DMSO) and aged (Alda1) vs young; ***Pvs young (WT) or Aged (DMSO) and aged (Alda1) vs young; #Pvs aged (WT) or aged (Alda1) vs aged (DMSO), ##Pvs aged (WT) or aged (Alda1) vs aged (DMSO).

Figure 11. The expression of apoptosis related proteins in aged ALDH2 overexpression and aged Alda1-treated mice. (A) A typical WB image of CHOP, caspase9 and caspase12 in aged ALDH2 overexpression mice; (BD) The expression of CHOP, caspase9 and caspase12 in aged ALDH2 overexpression mice; (E) A typical WB picture of CHOP, caspase9 and caspase12 in aged Alda1-treated mice; (FH) The expression of CHOP, caspase9 and caspase12 in aged Alda1-treated mice. All analyses were performed in duplicate. Values are presented as the mean ± SD, n = 4 mice per group. *P<0.05: aged (WT) and aged (ALDH2+) vs young (WT) or Aged (DMSO) and aged (Alda1) vs young; **P<0.01: aged (WT) and aged (ALDH2+) vs young (WT) or Aged (DMSO) and aged (Alda1) vs young; ***P<0.001: aged (WT) and aged (ALDH2+) vs young (WT) or Aged (DMSO) and aged (Alda1) vs young; #P<0.05: aged (ALDH2+) vs aged (WT) or aged (Alda1) vs aged (DMSO), ##P<0.01: aged (ALDH2+) vs aged (WT) or aged (Alda1) vs aged (DMSO).