Research Paper Volume 13, Issue 2 pp 2768—2779

PTPN2 negatively regulates macrophage inflammation in atherosclerosis

PTPN2 is negatively correlated with inflammation in ApoE-/- mice. (A) Photomicrograph of atherosclerotic lesions stained with Hematoxylin-Eosin. (B) The mRNA level of IL-6, IL-12 and IL-1β in macrophages from normal and inflamed aortic roots were analyzed by qRT-PCR. (C) ELISA assay analysis the production of IL-6, IL-12, IL-1β in macrophages from normal and inflamed fictions. (D) PTPN2 mRNA level in normal and inflamed aortic roots were analyzed by qRT-PCR. (E) IB analysis PTPN2 expression from three different ApoE-/- mice. M-1, M-2, M-3 were three different ApoE-/- mice. (F) Confocal microscopy analysis CD11b and PTPN2 in normal and inflamed aortic roots in ApoE-/- mice. Data is representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P

Figure 1. PTPN2 is negatively correlated with inflammation in ApoE-/- mice. (A) Photomicrograph of atherosclerotic lesions stained with Hematoxylin-Eosin. (B) The mRNA level of IL-6, IL-12 and IL-1β in macrophages from normal and inflamed aortic roots were analyzed by qRT-PCR. (C) ELISA assay analysis the production of IL-6, IL-12, IL-1β in macrophages from normal and inflamed fictions. (D) PTPN2 mRNA level in normal and inflamed aortic roots were analyzed by qRT-PCR. (E) IB analysis PTPN2 expression from three different ApoE-/- mice. M-1, M-2, M-3 were three different ApoE-/- mice. (F) Confocal microscopy analysis CD11b and PTPN2 in normal and inflamed aortic roots in ApoE-/- mice. Data is representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P < 0.05. **, P < 0.01.