Research Paper Volume 13, Issue 2 pp 2822—2850

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice

Silencing Rac1, p22-phox, p47-phox, or p67-phox promotes BMMSCs proliferation and viability after an OGD treatment. (A) Images representing Ki67 (green) expression in modified BMMSCs (transfected with shRac1, shP22, shP47, shP67, shLuci) and wild type BMMSCs, and counterstained with DAPI (blue). In comparison with the shLuci-transfected cells, other BMMSCs cell lines showed more Ki67(+) cells. The upper right corner insert shows a zoomed-in image of the local area with scale bar of 100μm. (B) The Ki67(+)/DAPI ratio was higher for BMMSCs transfected with shRac1, shP22, shP47, or shP67. (N.S. no significance, ****P C) BMMSCs viability, as measured by the CCK-8 assay, showed improved BMMSCs survival after transfection with shRac1, shP22, shP47, or shP67 lentivirus. (N.S. no significance, **P

Figure 2. Silencing Rac1, p22-phox, p47-phox, or p67-phox promotes BMMSCs proliferation and viability after an OGD treatment. (A) Images representing Ki67 (green) expression in modified BMMSCs (transfected with shRac1, shP22, shP47, shP67, shLuci) and wild type BMMSCs, and counterstained with DAPI (blue). In comparison with the shLuci-transfected cells, other BMMSCs cell lines showed more Ki67(+) cells. The upper right corner insert shows a zoomed-in image of the local area with scale bar of 100μm. (B) The Ki67(+)/DAPI ratio was higher for BMMSCs transfected with shRac1, shP22, shP47, or shP67. (N.S. no significance, ****P < 0.0001, statistically analyzed by one-way ANOVA followed by the Bonferroni correction, n = 4). (C) BMMSCs viability, as measured by the CCK-8 assay, showed improved BMMSCs survival after transfection with shRac1, shP22, shP47, or shP67 lentivirus. (N.S. no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001, statistically analyzed by one-way ANOVA followed by the Bonferroni comparison, n = 3). Data are presented as mean ± SD.