Research Paper Volume 13, Issue 2 pp 2822—2850

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice

shRac1 improves neuron survival by promoting BMMSCs transplant survival after transplantation into TBI mice. (A) Images representing surviving BMMSCs-shLuci and BMMSCs-shRac1 at 3, 7 and 21 DPT. Surviving BMMSCs in the lesional site were labeled with mCherry (red) and counterstained with DAPI (blue). Lesional boundaries were outlined by a continuous imaginary line. The upper right corner insert shows a zoomed-in image of the local area with a scale bar of 100μm. (B) mCherry (+) cell counts per field showed more BMMSCs-shRac1 cells survived than the BMMSCs-shLuci cells at day 3, day 7 and day 21 (*P C) Representative images of the surviving neurons at 3, 7 and 21 DPT, neuron surviving in the lesion location were labeled with NeuN (green) and counterstained with DAPI (blue). The upper right corner insert shows a zoomed-in image of the local area with scale bar of 100 μm. (D) NeuN (+)/DAPI ratio was higher for mice transplanted with BMMSCs-shRac1 than those transplanted with parental BMMSCs and BMMSCs-shLuci at different time points. (N.S. no significance, **P

Figure 5. shRac1 improves neuron survival by promoting BMMSCs transplant survival after transplantation into TBI mice. (A) Images representing surviving BMMSCs-shLuci and BMMSCs-shRac1 at 3, 7 and 21 DPT. Surviving BMMSCs in the lesional site were labeled with mCherry (red) and counterstained with DAPI (blue). Lesional boundaries were outlined by a continuous imaginary line. The upper right corner insert shows a zoomed-in image of the local area with a scale bar of 100μm. (B) mCherry (+) cell counts per field showed more BMMSCs-shRac1 cells survived than the BMMSCs-shLuci cells at day 3, day 7 and day 21 (*P < 0.05, **P < 0.01, ***P < 0.001, statistically analyzed by the Student’s t-test, n = 6). (C) Representative images of the surviving neurons at 3, 7 and 21 DPT, neuron surviving in the lesion location were labeled with NeuN (green) and counterstained with DAPI (blue). The upper right corner insert shows a zoomed-in image of the local area with scale bar of 100 μm. (D) NeuN (+)/DAPI ratio was higher for mice transplanted with BMMSCs-shRac1 than those transplanted with parental BMMSCs and BMMSCs-shLuci at different time points. (N.S. no significance, **P < 0.01, ***P < 0.001, #P < 0.05 versus parental BMMSCs transplanted group, statistically analyzed by Student’s t-test, n = 6). Data are presented as mean ± SD.