Research Paper Volume 13, Issue 2 pp 2864—2884

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis

The lncRNA GAS5 acts as a tumor suppressor in cisplatin-resistant NSCLC cells. (A) Expression of GAS5 in NSCLC and paired adjacent normal tissue samples was determined by qRT-PCR (n=41). (B) The expression of GAS5 in A549/DDP and H1299/DDP cells transduced with pcDNA-lncRNA GAS5 or pcDNA-NC was detected by qRT-PCR. (C) Cell viability of A549/DDP and H1299/DDP cells transfected with pcDNA-lncRNA GAS5 or pcDNA-NC was determined by CCK-8. (D) The invasion of A549/DDP and H1299/DDP cells transfected with pcDNA- lncRNA GAS5 or pcDNA-NC was determined by a transwell assay. *p

Figure 1. The lncRNA GAS5 acts as a tumor suppressor in cisplatin-resistant NSCLC cells. (A) Expression of GAS5 in NSCLC and paired adjacent normal tissue samples was determined by qRT-PCR (n=41). (B) The expression of GAS5 in A549/DDP and H1299/DDP cells transduced with pcDNA-lncRNA GAS5 or pcDNA-NC was detected by qRT-PCR. (C) Cell viability of A549/DDP and H1299/DDP cells transfected with pcDNA-lncRNA GAS5 or pcDNA-NC was determined by CCK-8. (D) The invasion of A549/DDP and H1299/DDP cells transfected with pcDNA- lncRNA GAS5 or pcDNA-NC was determined by a transwell assay. *p < 0.05, **p < 0.01, ***p < 0.001. The same experiments were performed at least three times.