Research Paper Volume 13, Issue 2 pp 2864—2884

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis

MiR-217 acts as a target of GAS5. (A) A schematic diagram of the sequence of miR-217 with GAS5 and GAS5 mutated at the putative binding site. (B) Relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with pcDNA- lncRNA GAS5 or pcDNA-NC measured by qRT-PCR. (C) The relative expression of GAS5 in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5-1 and si-GAS5-2 were detected by qRT-PCR. (D) The relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5-1 and si-GAS5-2 was detected by qRT-PCR. (E) The relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with pPG-miR-217, pPG-anti-miR-217 and pPG-miR-NC was quantified by qRT-PCR. (F) The relative expression of GAS5 in A549/DDP and H1299/DDP cells transfected with pPG-miR-217, pPG-anti-miR-217 and pPG-miR-NC was detected by qRT-PCR. *p

Figure 2. MiR-217 acts as a target of GAS5. (A) A schematic diagram of the sequence of miR-217 with GAS5 and GAS5 mutated at the putative binding site. (B) Relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with pcDNA- lncRNA GAS5 or pcDNA-NC measured by qRT-PCR. (C) The relative expression of GAS5 in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5-1 and si-GAS5-2 were detected by qRT-PCR. (D) The relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5-1 and si-GAS5-2 was detected by qRT-PCR. (E) The relative expression of miR-217 in A549/DDP and H1299/DDP cells transfected with pPG-miR-217, pPG-anti-miR-217 and pPG-miR-NC was quantified by qRT-PCR. (F) The relative expression of GAS5 in A549/DDP and H1299/DDP cells transfected with pPG-miR-217, pPG-anti-miR-217 and pPG-miR-NC was detected by qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001. The same experiments were performed at least three times.