Research Paper Volume 13, Issue 2 pp 2864—2884

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis

LHPP is located downstream of miR-217 and l GAS5. (A, B) Both qRT-PCR and Western blotting were used to determine the relative mRNA and protein expression of LHPP in A549/DDP and H1299/DDP cells transfected with pPG-miR-NC, pPG-miR-217 and pPG-anti-miR-217. (C, D) qRT-PCR and Western blotting were used to determine the relative mRNA and protein levels of LHPP in A549/DDP and H1299/DDP cells transfected with pcDNA-NC, pcDNA-GAS5 or pcDNA-GAS5 + pPG-miR-217. (E, F) qRT-PCR and Western blotting were used to determine the relative mRNA and protein levels of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5 or si-GAS5 + pPG-anti-miR-217. *p

Figure 4. LHPP is located downstream of miR-217 and l GAS5. (A, B) Both qRT-PCR and Western blotting were used to determine the relative mRNA and protein expression of LHPP in A549/DDP and H1299/DDP cells transfected with pPG-miR-NC, pPG-miR-217 and pPG-anti-miR-217. (C, D) qRT-PCR and Western blotting were used to determine the relative mRNA and protein levels of LHPP in A549/DDP and H1299/DDP cells transfected with pcDNA-NC, pcDNA-GAS5 or pcDNA-GAS5 + pPG-miR-217. (E, F) qRT-PCR and Western blotting were used to determine the relative mRNA and protein levels of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-GAS5 or si-GAS5 + pPG-anti-miR-217. *p < 0.05, **p < 0.01, ***p < 0.001. The same experiments were performed at least three times.