Research Paper Volume 13, Issue 2 pp 2864—2884

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis

LHPP serves as a tumor suppressor in cisplatin-resistant NSCLC cells. (A) The relative mRNA expression of LHPP in NSCLC tissues and their paired adjacent normal tissues was determined by qRT-PCR. (B) The relative mRNA expression of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-LHPP-1 and si-LHPP-2 was measured by qRT-PCR. (C) The relative protein expression of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-LHPP-1 and si-LHPP-2 was measured by Western blotting. (D) The relative protein expression of E-cadherin, Vimentin, Twist and PCNA in A549/DDP and H1299/DDP cells was measured by Western blotting after silencing of LHPP by si-LHPP. (E) The cloning ability of A549/DDP and H1299/DDP cells was determined by colony formation assays after silencing of LHPP by si-LHPP. (F) The invasion ability of A549/DDP and H1299/DDP cells was determined by transwell invasion assays after silencing LHPP by si-LHPP. *p

Figure 5. LHPP serves as a tumor suppressor in cisplatin-resistant NSCLC cells. (A) The relative mRNA expression of LHPP in NSCLC tissues and their paired adjacent normal tissues was determined by qRT-PCR. (B) The relative mRNA expression of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-LHPP-1 and si-LHPP-2 was measured by qRT-PCR. (C) The relative protein expression of LHPP in A549/DDP and H1299/DDP cells transfected with si-NC, si-LHPP-1 and si-LHPP-2 was measured by Western blotting. (D) The relative protein expression of E-cadherin, Vimentin, Twist and PCNA in A549/DDP and H1299/DDP cells was measured by Western blotting after silencing of LHPP by si-LHPP. (E) The cloning ability of A549/DDP and H1299/DDP cells was determined by colony formation assays after silencing of LHPP by si-LHPP. (F) The invasion ability of A549/DDP and H1299/DDP cells was determined by transwell invasion assays after silencing LHPP by si-LHPP. *p < 0.05, **p < 0.01, ***p < 0.001. Colony formation assays were performed in three replicates.