Figure 8. External validation of SNRPA1 in molecular experiments. Representative immunoblot (A) and quantification (B) of SNRPA1 expression between PCa and normal clinical samples. Representative immunoblot (C) and quantification (D) of SNRPA1 expression after shRNA transfection in CWR22Rv1 and C4-2b cells. Representative results of scratch assay (E) and quantification (F) of CWR22Rv1 and C4-2b cells. Representative results of colony formation (G) and quantification (H) of CWR22Rv1 and C4-2b cells. (I) The results of cell proliferation using cell counting kit-8 of CWR22Rv1 and C4-2b cells after 48 hours. Data are expressed as means ± SD. *P <0.05. PCa: Prostate cancer; N: normal sample; T: tumor sample; shRNA: short hairpin RNAs; shNC: negative control shRNA; shSNRPA1: shRNA targeting SNRPA1.