Research Paper Volume 13, Issue 2 pp 2941—2958
Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2
Figure 4. Effects of Notch2 on biological functions of gallbladder carcinoma cells. (A) Expression of Notch2 in GBC-SD and NOZ cells after miR transfection was detected by western blot; (B) Proliferation of GBC-SD and NOZ cells after transfection was measured by CCK-8 assay; (C) Membrane penetration of GBC-SD and NOZ cells after transfection was measured by transwell assay; (D) Apoptosis rates of GBC-SD and NOZ cells after transfection by flow cytometry; (E) Migration of GBC-SD and NOZ cells after transfection was analyzed by cell scratch assay; (F) Expression of Vimentin, N-cadherin and E-cadherin, in GBC-SD and NOZ cells after transfection by western blot. a indicates in comparison with the si-NC group, p< 0.05.