Research Paper Volume 13, Issue 2 pp 2941—2958

Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2

Effects of Notch2 on biological functions of gallbladder carcinoma cells. (A) Expression of Notch2 in GBC-SD and NOZ cells after miR transfection was detected by western blot; (B) Proliferation of GBC-SD and NOZ cells after transfection was measured by CCK-8 assay; (C) Membrane penetration of GBC-SD and NOZ cells after transfection was measured by transwell assay; (D) Apoptosis rates of GBC-SD and NOZ cells after transfection by flow cytometry; (E) Migration of GBC-SD and NOZ cells after transfection was analyzed by cell scratch assay; (F) Expression of Vimentin, N-cadherin and E-cadherin, in GBC-SD and NOZ cells after transfection by western blot. a indicates in comparison with the si-NC group, p

Figure 4. Effects of Notch2 on biological functions of gallbladder carcinoma cells. (A) Expression of Notch2 in GBC-SD and NOZ cells after miR transfection was detected by western blot; (B) Proliferation of GBC-SD and NOZ cells after transfection was measured by CCK-8 assay; (C) Membrane penetration of GBC-SD and NOZ cells after transfection was measured by transwell assay; (D) Apoptosis rates of GBC-SD and NOZ cells after transfection by flow cytometry; (E) Migration of GBC-SD and NOZ cells after transfection was analyzed by cell scratch assay; (F) Expression of Vimentin, N-cadherin and E-cadherin, in GBC-SD and NOZ cells after transfection by western blot. a indicates in comparison with the si-NC group, p< 0.05.