Figure 2. Early-life GH intervention effects brain expression of DNMTs and H3 methylation on lysine 4 and 27 in Ames dwarf mice. (A, B) Brain DNMTs expression is shown for 20-month-old males (A) and females (B). (C, F) Representative western blots in males (C) and females (F). (D, G) Quantification of brain H3K4me3 and H3K27me3 in Ames dwarf males (D) and females (G) mice upon early-life GH intervention. (E, H) mRNA level of EZH2 in males (E) and females (H) of Ames dwarf mice. mRNA analysis data (means ± sem) (A, B, E–H) are normalized to GAPDH and expressed as fold change compared with WT control (defined as 1.0). Protein quantification data (means ± SEM) (D, G) are normalized to histone H3 and expressed as fold change compared with WT control (defined as 1.0). n=6 mice for each group. Data are means ± SEM. * p < 0.05, ** p < 0.01 by one-way ANOVA.