Research Paper Volume 13, Issue 2 pp 3060—3079

Human umbilical cord mesenchymal stem cell-derived exosomal miR-146a-5p reduces microglial-mediated neuroinflammation via suppression of the IRAK1/TRAF6 signaling pathway after ischemic stroke

Analysis of human umbilical mesenchymal stem cells (hUMSCs) and hUMSC-derived exosomes (hUMSC-Exos). (A) Representative micrographs of cultured hUMSCs at passage 3 (P3). Scale bar: 200 μm. (B) Flow cytometry analysis of hUMSC CD73, CD105, CD90, CD11b, CD19, CD34, CD45, and HLA-DR expression. (C) Representative electron micrographs of hUMSC-Exos. Scale bar: 200 nm. (D) Exosome particle size and concentration. (E) Western blot analysis of Exos-specific markers CD9, ALIX, and TSG101. Each blot represents three independent experiments of two samples each.

Figure 1. Analysis of human umbilical mesenchymal stem cells (hUMSCs) and hUMSC-derived exosomes (hUMSC-Exos). (A) Representative micrographs of cultured hUMSCs at passage 3 (P3). Scale bar: 200 μm. (B) Flow cytometry analysis of hUMSC CD73, CD105, CD90, CD11b, CD19, CD34, CD45, and HLA-DR expression. (C) Representative electron micrographs of hUMSC-Exos. Scale bar: 200 nm. (D) Exosome particle size and concentration. (E) Western blot analysis of Exos-specific markers CD9, ALIX, and TSG101. Each blot represents three independent experiments of two samples each.