COVID-19 Research Paper Volume 13, Issue 2 pp 1571—1590

Fighting the storm: could novel anti-TNFα and anti-IL-6 C. sativa cultivars tame cytokine storm in COVID-19?

Effects of novel C. sativa extracts on the levels of IL-6 and COX2 in WI-38 lung fibroblasts. WI-38 cells grown to 80% confluency were treated with either 10 ng/ml TNFα /IFN γ alone or in combination with the indicated extracts; at 48 h after treatment, the whole cellular lysates were prepared and subjected to Western blot analysis using antibodies against IL-6 and COX2 as detailed in “Methods”. GAPDH served as a loading control.

Figure 4. Effects of novel C. sativa extracts on the levels of IL-6 and COX2 in WI-38 lung fibroblasts. WI-38 cells grown to 80% confluency were treated with either 10 ng/ml TNFα /IFN γ alone or in combination with the indicated extracts; at 48 h after treatment, the whole cellular lysates were prepared and subjected to Western blot analysis using antibodies against IL-6 and COX2 as detailed in “Methods”. GAPDH served as a loading control.