Figure 1. Sleep deprivation aggravates brain damage after SAH. (A) Brain water content assay shows that sleep deprivation aggravated brain edema after SAH. (B, C) TUNEL staining (B) and quantification (C) show that sleep deprivation increased apoptosis after SAH. Scale bar, 50 μm. (D) Western blot shows that sleep deprivation increased expression of cleaved-caspase-3 (C-caspase-3) after SAH. The data was represented as means ± SEM. #p < 0.05 vs. Sham group and *p < 0.05 vs. SAH group.