Research Paper Volume 13, Issue 4 pp 5875—5891

Blockade of Indoleamine 2, 3-dioxygenase 1 ameliorates hippocampal neurogenesis and BOLD-fMRI signals in chronic stress precipitated depression

Inhibition of IDO1 activity in the DRN improved the BDNF expression and neurogenesis in the dentate gyrus of hippocampus after CUMS treatment. (A) Western blot analysis of BDNF in the hippocampus of Control, CUMS, Control + INCB024360 and CUMS + INCB024360 groups. (B) IF analysis of the numbers of Nestin-positive stem cell in the dentate gyrus of hippocampus after INCB024360 treatment (scale bar 100 μm). n = 6 mice/group. Bars represent mean±SEM. *ppp

Figure 3. Inhibition of IDO1 activity in the DRN improved the BDNF expression and neurogenesis in the dentate gyrus of hippocampus after CUMS treatment. (A) Western blot analysis of BDNF in the hippocampus of Control, CUMS, Control + INCB024360 and CUMS + INCB024360 groups. (B) IF analysis of the numbers of Nestin-positive stem cell in the dentate gyrus of hippocampus after INCB024360 treatment (scale bar 100 μm). n = 6 mice/group. Bars represent mean±SEM. *p< 0.05, ** p< 0.01, *** p< 0.001.