Research Paper Volume 13, Issue 2 pp 1649—1670

Sulforaphane promotes C. elegans longevity and healthspan via DAF-16/DAF-2 insulin/IGF-1 signaling

Low-dose sulforaphane does not inhibit OP50 bacterial growth and C. elegans food preferences. (A) A single colony of E. coli OP50 was allowed to grow in 3 ml of LB broth medium in a 37° C shaker overnight. The overnight culture was diluted to an OD600 of approximately 0.1. Then, 15 ml aliquots of this dilution were pipetted into 50 ml falcon tubes in the presence or absence of 100 μM, 200 μM, 300 μM, or 400 μM sulforaphane (SF) as indicated, followed by incubation in a 37° C shaker. The OD600 was measured every 30 min up to 8 h, and the resulting growth curves are presented as E. coli OP50 growth (OD600). (B) Fifty wild-type L4 larvae were placed in the center of an NGM plate with E. coli OP50 bacteria, where the bacteria on the left side contained 100 μM sulforaphane (SF), whereas those on the right side did not (CO), as indicated in the scheme on the left. The number of C. elegans worms on each side of the agar plate was determined by picking and counting the nematodes 3 h and 6 h later. The worm fraction was calculated as the ratio of the number of worms crawling on each side of the plate. The initial number of 50 worms was set as 1. The data are presented in the diagram on the right. Mean values ±SD are given.

Figure 2. Low-dose sulforaphane does not inhibit OP50 bacterial growth and C. elegans food preferences. (A) A single colony of E. coli OP50 was allowed to grow in 3 ml of LB broth medium in a 37° C shaker overnight. The overnight culture was diluted to an OD600 of approximately 0.1. Then, 15 ml aliquots of this dilution were pipetted into 50 ml falcon tubes in the presence or absence of 100 μM, 200 μM, 300 μM, or 400 μM sulforaphane (SF) as indicated, followed by incubation in a 37° C shaker. The OD600 was measured every 30 min up to 8 h, and the resulting growth curves are presented as E. coli OP50 growth (OD600). (B) Fifty wild-type L4 larvae were placed in the center of an NGM plate with E. coli OP50 bacteria, where the bacteria on the left side contained 100 μM sulforaphane (SF), whereas those on the right side did not (CO), as indicated in the scheme on the left. The number of C. elegans worms on each side of the agar plate was determined by picking and counting the nematodes 3 h and 6 h later. The worm fraction was calculated as the ratio of the number of worms crawling on each side of the plate. The initial number of 50 worms was set as 1. The data are presented in the diagram on the right. Mean values ±SD are given.