Research Paper Volume 13, Issue 2 pp 1649—1670

Sulforaphane promotes C. elegans longevity and healthspan via DAF-16/DAF-2 insulin/IGF-1 signaling

DAF-2/insulin/IGF-1 signaling is required for sulforaphane-mediated longevity. (A) Schematic representation of signaling cascades that are known to regulate longevity in C. elegans, together with the names of the respective mutant C. elegans strains are shown, e.g., reduced DAF-2/insulin signaling, reduced eat-2/sir-2.1 dietary restriction signaling, and enhanced isp-1 mitochondrial respiration. (B) Kaplan-Meier survival curves were generated using C. elegans strains with mutations in daf-2, eat-2, sir-2.1 and isp-1. The worms were fed with OP50 bacterial only (CO) or with 100 μM sulforaphane plus OP50 bacteria (SF), as described in Fig. 1. The daf-2 mutant daf-2(e1370) III strain was cultivated at 15° C, and all other strains were cultivated at 20° C. daf-2(e1370) III: P>0.05, eat-2(ad1113) II: Psir-2.1(ok434) IV: Pisp-1(qm150) IV(D): P

Figure 5. DAF-2/insulin/IGF-1 signaling is required for sulforaphane-mediated longevity. (A) Schematic representation of signaling cascades that are known to regulate longevity in C. elegans, together with the names of the respective mutant C. elegans strains are shown, e.g., reduced DAF-2/insulin signaling, reduced eat-2/sir-2.1 dietary restriction signaling, and enhanced isp-1 mitochondrial respiration. (B) Kaplan-Meier survival curves were generated using C. elegans strains with mutations in daf-2, eat-2, sir-2.1 and isp-1. The worms were fed with OP50 bacterial only (CO) or with 100 μM sulforaphane plus OP50 bacteria (SF), as described in Fig. 1. The daf-2 mutant daf-2(e1370) III strain was cultivated at 15° C, and all other strains were cultivated at 20° C. daf-2(e1370) III: P>0.05, eat-2(ad1113) II: P< 0.001, sir-2.1(ok434) IV: P< 0.01, isp-1(qm150) IV(D): P< 0.001.