Research Paper Volume 13, Issue 5 pp 6712—6723

CPI-1189 protects neuronal cells from oxygen glucose deprivation/re-oxygenation-induced oxidative injury and cell death

CPI-1189 inhibits OGDR-induced apoptosis activation in neuronal cells. SH-SY5Y neuronal cells (A–F, I–K) or primary murine cortical neurons (G, H, J–L) were pretreated for 1h with CPI-1189 (100 nM) and stimulated with OGDR or hydrogen peroxide (H2O2, 300 μM), cells were cultured for applied time periods, caspase activation and cell apoptosis were tested by the assays mentioned in the text. Quantified values were mean ± standard deviation (SD, n=5). * P vs. “Mock” cells. #P vs. cells with OGDR stimulation/H2O2 treatment but “DMSO (0.1%)” pretreatment. Experiments were repeated three times, with similar results obtained. Scale bar= 100 μm (E).

Figure 3. CPI-1189 inhibits OGDR-induced apoptosis activation in neuronal cells. SH-SY5Y neuronal cells (AF, IK) or primary murine cortical neurons (G, H, JL) were pretreated for 1h with CPI-1189 (100 nM) and stimulated with OGDR or hydrogen peroxide (H2O2, 300 μM), cells were cultured for applied time periods, caspase activation and cell apoptosis were tested by the assays mentioned in the text. Quantified values were mean ± standard deviation (SD, n=5). * P < 0.05 vs. “Mock” cells. #P < 0.05 vs. cells with OGDR stimulation/H2O2 treatment but “DMSO (0.1%)” pretreatment. Experiments were repeated three times, with similar results obtained. Scale bar= 100 μm (E).