Research Paper Volume 13, Issue 5 pp 6724—6739

MiR-506 exerts antineoplastic effects on osteosarcoma cells via inhibition of the Skp2 oncoprotein

Upregulation of miR-506 suppresses cell viability. (A) The miR-506 level was evaluated by RT-PCR in osteosarcoma cells transfected with miR-506 mimics. *p B) Cell viability was detected by MTT method in MG63 and U2OS cells after miR-506 overexpression. (C) Left panel: Apoptosis was evaluated by flow cytometry in osteosarcoma cells after miR-506 mimic transfection. Right panel: Quantification of apoptosis results.

Figure 1. Upregulation of miR-506 suppresses cell viability. (A) The miR-506 level was evaluated by RT-PCR in osteosarcoma cells transfected with miR-506 mimics. *p < 0.05, compared to control. (B) Cell viability was detected by MTT method in MG63 and U2OS cells after miR-506 overexpression. (C) Left panel: Apoptosis was evaluated by flow cytometry in osteosarcoma cells after miR-506 mimic transfection. Right panel: Quantification of apoptosis results.